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Although the antibody directed mMEC purification method allowed the specific selection of epithelial cells, which account for less than 5% of total milk cells (SC), the mMEC transcriptome was different from the rest of the transcriptomes analyzed. In our results, immunomagnetic mMEC purification method showed that a conjoint of reactions was affecting the proportion and/or the function of white cells present in milk, showing a unique set of highly expressed genes such as TUBA1B, HMOX1, S100A11 and RL10 involved in anti inflammatory processes, cell death or apoptosis, specifically in Jersey mMEC samples.Up to this point, the majority of gene expression analyses related to bovine lactation have been developed using tissues obtained by biopsy22,23. This biopsy approach is invasive during a stage when vascularization of the gland is at a maximum, which presents significant technical challenges and management issues in the recovery of the animals.An alternative sampling procedure has been proposed via isolating mRNA directly from mMEC8,9, MFG11 and SC6,7 that are released into milk during lactation.
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